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Sample GSM828663 Query DataSets for GSM828663
Status Public on Jun 18, 2012
Title Keratinocytes_R500P_rep2
Sample type RNA
Source name Primary neonatal keratinocytes
Organism Homo sapiens
Characteristics cell type: primary neonatal keratinocytes
genotype/variation: ΔNp63α(R500P) Overexpression
Treatment protocol siRNA knock down
Growth protocol Keratinocytes were grown in Gibco SFM medium till 100% confluent with addition of 1.2 mM Ca2+ for 3 Days to induce differentiation.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kits were used to isolate total RNA.
Label biotin
Label protocol Affymetrix 3' IVT Express Kit
Hybridization protocol Affymetrix GeneChip Hybridization, Wash, and Stain Kit
Scan protocol Affymetrix GeneChip Scanner 3000 7G
Description replicate 2
Data processing RMA normalization using justRMA package in R 2.11.1
Submission date Nov 04, 2011
Last update date Oct 11, 2022
Contact name Douglas Porter
Organization name Stanford
Department Dermatology
Lab Khavari
Street address 269 Campus Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platform ID GPL570
Series (2)
GSE33495 Disrupted transcripitonal network in ΔNp63 AEC tissue model [gene expression]
GSE33572 Disrupted transcripitonal network in ΔNp63 AEC tissue model

Data table header descriptions
VALUE RNA-normalized value

Data table
1007_s_at 10.86399817
1053_at 7.707504515
117_at 5.041915369
121_at 7.433534757
1255_g_at 3.368205078
1294_at 5.559016384
1316_at 5.569884525
1320_at 5.542649693
1405_i_at 3.815882725
1431_at 4.003929544
1438_at 7.154237441
1487_at 8.074545993
1494_f_at 6.035262756
1552256_a_at 8.565058466
1552257_a_at 10.17035997
1552258_at 5.274358123
1552261_at 4.534814298
1552263_at 7.230368992
1552264_a_at 8.058350032
1552266_at 3.371717997

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM828663.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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