|
| Status |
Public on Nov 09, 2011 |
| Title |
AtGATA2ox, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Overexpression line of AtGATA2 in Columbia background
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia-0 (Col-0)
genotype/variation: AtGATA2ox transgenic
tissue: seedling
|
| Treatment protocol |
The seedlings were frozen in liquid nitrogen in complete darkness, and then the homozygote seedlings of bri1-116 were picked individually from the heterozygous population.
|
| Growth protocol |
Seedlings were grown on solid ½ MS medium with 1% sucrose in the dark for 108 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by the Plant RNA Purification Reagent (Invitrogen), treated with RNase-free DNase I (Qiagen), and cleaned up using the RNeasy Plant Mini column (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared from 10ug of total RNA according to the Eukaryotic Target Protocol (Affymetrix).
|
| |
|
| Hybridization protocol |
20 μg of labeled and fragmented cRNA were hybridized with the Arabidopsis ATH1 genome array according to the manufacturer’s instructions (Affymetrix).
|
| Scan protocol |
Arrays were scanned using the Agilent GeneArray Scanner.
|
| Description |
Gene expression data from overexpression line of AtGATA2 gene, repeat 1.
|
| Data processing |
Microarray data were analyzed using Genespring software (version 7, Silicon Genetics). Data that were flagged as absent using mismatch probes were removed from the analysis. GC-RMA signal intensities were generated. However, note that MAS5.0 signal intensities are presented in the table below. ANOVA was used to find genes whose expression was different between AtGATA2ox and Col or between AtGATA2ox and bri1-116.
|
| |
|
| Submission date |
Nov 09, 2011 |
| Last update date |
Nov 09, 2011 |
| Contact name |
wenhui lin |
| E-mail(s) |
whlin@ibcas.ac.cn
|
| Organization name |
Institute of Botany, CAS
|
| Street address |
No.20 Nanxincun, Xiangshan
|
| City |
Beijing |
| ZIP/Postal code |
100093 |
| Country |
China |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE25396 |
Expression data from dark-grown AtGATA2 (At2g45050) overexpression transgenic line, homozygous bri1-116 and Col-0 wild type |
|
