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Sample GSM831605 Query DataSets for GSM831605
Status Public on Jun 04, 2013
Title MelJuSo cells 2 days post doxorubicin
Sample type RNA
 
Source name MelJuSo cells 2 days post doxorubicin
Organism Homo sapiens
Characteristics cell line: MelJuSo
cell type: Human skin melanoma cell line
treatment: Doxorubicin 9uM 2 hours
time point: 2 days post drug removal
Treatment protocol Cells were treated with indicated drugs for 2 hours, followed by entensive washing to remove the drug.
Growth protocol Cells were cultured in 10cm dishes and reached 80% confluency before treatment.
Extracted molecule total RNA
Extraction protocol Trizol extraction + RNeasy clean-up with DNase treatment (Qiagen)
Label biotin
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol
Scan protocol Standard Illumina protocol
Data processing Beadlevel data was processed using the beadarray package, affy model background corrected, log2 transformed and RSN normalized using the lumi package. All packages are available in Bioconductor.
 
Submission date Nov 11, 2011
Last update date Jun 04, 2013
Contact name Baoxu Pang
Organization name Stanford University
Department Genetics
Lab Michael Snyder
Street address 3165 Porter Drive
City Palo Alto
State/province CALIFORNIA
ZIP/Postal code 94304
Country USA
 
Platform ID GPL13376
Series (2)
GSE33624 Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 1)
GSE33634 Topoisomerase II inhibitors and histone eviction

Data table header descriptions
ID_REF
VALUE Log2 transformed RSN normalized.
Detection_Pvalue

Data table
ID_REF VALUE Detection_Pvalue
ILMN_1725528 6.956960592 0
ILMN_1683555 2.4688623 0.196507
ILMN_1818771 2.433294464 0.208151
ILMN_1773680 2.582290054 0.166667
ILMN_1731647 5.044161779 0.00800582
ILMN_1845201 1.873048958 0.496361
ILMN_1746533 5.716938603 0.00218341
ILMN_1800540 8.420776583 0
ILMN_1881186 1.106090589 0.968705
ILMN_1878595 1.566091246 0.724163
ILMN_1876148 1.575090148 0.718341
ILMN_1915152 1.788066699 0.56623
ILMN_1886710 2.461869556 0.19869
ILMN_1848344 2.141265113 0.329694
ILMN_1785340 5.887808321 0.00218341
ILMN_1779536 5.589526238 0.00218341
ILMN_1872584 1.409507783 0.830422
ILMN_1915587 2.530904303 0.179039
ILMN_1654552 9.565821124 0
ILMN_1754400 8.064607609 0

Total number of rows: 48701

Table truncated, full table size 1571 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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