|
Status |
Public on Jun 04, 2013 |
Title |
MelJuSo cells 2 days post doxorubicin |
Sample type |
RNA |
|
|
Source name |
MelJuSo cells 2 days post doxorubicin
|
Organism |
Homo sapiens |
Characteristics |
cell line: MelJuSo cell type: Human skin melanoma cell line treatment: Doxorubicin 9uM 2 hours time point: 2 days post drug removal
|
Treatment protocol |
Cells were treated with indicated drugs for 2 hours, followed by entensive washing to remove the drug.
|
Growth protocol |
Cells were cultured in 10cm dishes and reached 80% confluency before treatment.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction + RNeasy clean-up with DNase treatment (Qiagen)
|
Label |
biotin
|
Label protocol |
Standard Illumina protocol
|
|
|
Hybridization protocol |
Standard Illumina protocol
|
Scan protocol |
Standard Illumina protocol
|
Data processing |
Beadlevel data was processed using the beadarray package, affy model background corrected, log2 transformed and RSN normalized using the lumi package. All packages are available in Bioconductor.
|
|
|
Submission date |
Nov 11, 2011 |
Last update date |
Jun 04, 2013 |
Contact name |
Baoxu Pang |
Organization name |
Stanford University
|
Department |
Genetics
|
Lab |
Michael Snyder
|
Street address |
3165 Porter Drive
|
City |
Palo Alto |
State/province |
CALIFORNIA |
ZIP/Postal code |
94304 |
Country |
USA |
|
|
Platform ID |
GPL13376 |
Series (2) |
GSE33624 |
Distinct effects of topoisomerase II inhibitors on tumor cell lines (part 1) |
GSE33634 |
Topoisomerase II inhibitors and histone eviction |
|