|
Status |
Public on Dec 01, 2011 |
Title |
CoCl2 sup35 1 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
BYxRM diploid hybrid
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
genetic background: BYxRM diploid hybrid selection: none genotype: wild type
|
Growth protocol |
Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
|
Label |
Cy5
|
Label protocol |
DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
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|
|
Channel 2 |
Source name |
BYxRM sup35 selected segregant on CoCl2
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
sample type: Pooled BYxRM sup35 segregant grown on YPD+CoCl2 biological replicate1 genetic background: BYxRM selection: Pooled BYxRM sup35 segregant grown on YPD+CoCl2 genotype: BYxRM sup35 segregant
|
Growth protocol |
Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
|
Label |
Cy3
|
Label protocol |
DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
|
|
|
|
Hybridization protocol |
Hybridization buffer (Agilent In Situ Hybridization Kit Plus) was added and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
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|
|
Submission date |
Nov 18, 2011 |
Last update date |
Dec 01, 2011 |
Contact name |
Noorossadat Torabi |
E-mail(s) |
ntorabi@Princeton.EDU
|
Organization name |
Princeton University
|
Department |
Molecular Biology
|
Lab |
Kruglyak
|
Street address |
110 Carl Icahn Lab
|
City |
Princeton |
State/province |
NJ |
ZIP/Postal code |
08544 |
Country |
USA |
|
|
Platform ID |
GPL14905 |
Series (1) |
GSE33817 |
X-QTL performed on wild-type and sup35 (C653R) BY x RM segregants in various growth conditions |
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