NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM836282 Query DataSets for GSM836282
Status Public on Dec 01, 2011
Title Cycloheximide sup35 1
Sample type genomic
 
Channel 1
Source name BYxRM diploid hybrid
Organism Saccharomyces cerevisiae
Characteristics genetic background: BYxRM diploid hybrid
selection: none
genotype: wild type
Growth protocol Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
Extracted molecule genomic DNA
Extraction protocol DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
Label Cy5
Label protocol DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
 
Channel 2
Source name BYxRM sup35 selected segregant on Cycloheximide
Organism Saccharomyces cerevisiae
Characteristics sample type: Pooled BYxRM sup35 segregant grown on YPD+Cycloheximide biological replicate1
genetic background: BYxRM
selection: Pooled BYxRM sup35 segregant grown on YPD+Cycloheximide
genotype: BYxRM sup35 segregant
Growth protocol Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
Extracted molecule genomic DNA
Extraction protocol DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
Label Cy3
Label protocol DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
 
 
Hybridization protocol Hybridization buffer (Agilent In Situ Hybridization Kit Plus) was added and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
Scan protocol Scanned on an Agilent G2565AA scanner.
Data processing Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
 
Submission date Nov 18, 2011
Last update date Dec 01, 2011
Contact name Noorossadat Torabi
E-mail(s) ntorabi@Princeton.EDU
Organization name Princeton University
Department Molecular Biology
Lab Kruglyak
Street address 110 Carl Icahn Lab
City Princeton
State/province NJ
ZIP/Postal code 08544
Country USA
 
Platform ID GPL14905
Series (1)
GSE33817 X-QTL performed on wild-type and sup35 (C653R) BY x RM segregants in various growth conditions

Data table header descriptions
ID_REF
VALUE For a given SNP, the difference in the log10 ratios of BY and RM-specific probes on a single array (or log10 intensity difference) was computed

Data table
ID_REF VALUE
chr1_27915Bd 0.204405318
chr1_28323Bd 0.123106559
chr1_28354Bu 0.239830368
chr1_28846Bu 0.08169245
chr1_29667Bd 0
chr1_29667Bu 0.066084701
chr1_30714Bu 0.000716
chr1_31059Bu 0.306340121
chr1_31213Bd 0.216585662
chr1_31636Bd 0.039258956
chr1_31756Bu 0.102423286
chr1_31849Bd 0.120463585
chr1_32512Bd -0.066962989
chr1_32512Bu 0.069885979
chr1_32653Bd 0.116742104
chr1_32653Bu 0.083632967
chr1_33201Bu 0.089308421
chr1_33294Bu 0.160450298
chr1_33549Bu 0.189803259
chr1_33613Bd 0.147024218

Total number of rows: 21994

Table truncated, full table size 572 Kbytes.




Supplementary file Size Download File type/resource
GSM836282_Cycloheximide_sup35_1.txt.gz 12.7 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap