|
| Status |
Public on Dec 01, 2011 |
| Title |
Diamide sup35 SKY1 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
BYxRM diploid hybrid
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genetic background: BYxRM diploid hybrid
selection: none
genotype: wild type
|
| Growth protocol |
Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
|
| Label |
Cy5
|
| Label protocol |
DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
|
| |
|
| Channel 2 |
| Source name |
BYxRM(SKY1BY) sup35 selected segregant on Diamide
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
sample type: Pooled BYxRM(SKY1BY) sup35 segregant grown on YPD+Diamide
genetic background: BYxRM
selection: Pooled BYxRM(SKY1BY) sup35 segregant grown on YPD+Diamide
genotype: BYxRM(SKY1BY) sup35 segregant
|
| Growth protocol |
Pooled Mata segregants from either wt or sup35 BYxRM cross were plated on YPD alone(control) or YPD+drug (selection) plates and were incubated at 30C for two days.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted from the grown cells using Genomic-tip 100/G columns (Qiagen; 10243)
|
| Label |
Cy3
|
| Label protocol |
DNA was labeled using the BioPrime Array CGH Genomic Labeling Module (Invitrogen; 18095-012) with the sample being labeled with Cy3 dUTP and the reference being labeled with Cy5 dUTP. We used a BY/RM diploid as the reference for all hybridizations.
|
| |
|
| |
| Hybridization protocol |
Hybridization buffer (Agilent In Situ Hybridization Kit Plus) was added and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Scanned on an Agilent G2565AA scanner.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version A.8.5.1.1).
|
| |
|
| Submission date |
Nov 18, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Noorossadat Torabi |
| E-mail(s) |
ntorabi@Princeton.EDU
|
| Organization name |
Princeton University
|
| Department |
Molecular Biology
|
| Lab |
Kruglyak
|
| Street address |
110 Carl Icahn Lab
|
| City |
Princeton |
| State/province |
NJ |
| ZIP/Postal code |
08544 |
| Country |
USA |
| |
|
| Platform ID |
GPL14905 |
| Series (1) |
| GSE33817 |
X-QTL performed on wild-type and sup35 (C653R) BY x RM segregants in various growth conditions |
|
