|
| Status |
Public on Dec 05, 2011 |
| Title |
normal stem cells rep5 |
| Sample type |
RNA |
| |
|
| Source name |
normal stem cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
age: Postnatal day 5-7
cell source: normal (uninfected) cerebellar stem cells
cell type: FACS-sorted Prominin1+, Lineage-negative (Lin–) cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
To isolate cerebellar stem cells, Prominin1+, Lineage-negative (Lin–) cells were FACS-sorted from cerebella of 5-7-day-old WT mice. Tumor cells were isolated by enzymatic dissociation of cerebella from adult tumor-bearing mice. All cells were snap-frozen in liquid nitrogen. RNA was isolated using the RNAqueous kit (Ambion), and was labeled and hybridized to Affymetrix Mouse Genome 430 2.0 arrays.
|
| Label |
biotin
|
| Label protocol |
As suggested by Affymetrix
|
| |
|
| Hybridization protocol |
As recommended by Affymetrix
|
| Scan protocol |
Using standard protocols
|
| Description |
Nsc_5
|
| Data processing |
Microarray data were preprocessed using robust multichip analysis (RMA) in Partek Genomics Suite 6.5 (Partek, Inc.). Result is log2 transformed normalized data.
|
| |
|
| Submission date |
Dec 03, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Alexey M Eroshkin |
| E-mail(s) |
eroshkin@sbpdiscovery.org
|
| Phone |
8584497516
|
| Organization name |
SBPMDI
|
| Department |
Bioinformatics Core
|
| Street address |
Alexey Eroshkin, 3803 Ruette San Raphael
|
| City |
SAN DIEGO |
| State/province |
California |
| ZIP/Postal code |
92130 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE34126 |
An Animal Model of Myc-driven medulloblastoma |
|
| Relations |
| Reanalyzed by |
GSE123409 |
