|
| Status |
Public on Dec 05, 2011 |
| Title |
8542_Dox_24hr_1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse mammary tumor derived cell line
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N;B6
gender: female
tissue: Primary mammary tumor
cell line: mouse mammary tumor derived cell line 8542
agent: doxycycline
intracellular domain of notch1 (icn1) status: Off
|
| Treatment protocol |
Cells were left untreated or treated with 2 ug/ml doxycycline for 24 hours.
|
| Growth protocol |
Cells were maintained in DMEM/F12 1:1 media supplemented with 10% fetal bovine serum (FBS), 2mM L-glutamine, penicillin (100 U/ml) and streptomycin (100 µg/ml). Cells were maintained at 37°C in an atmosphere of 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected by scraping and total RNA was isolated using Trizol (Invitrogen), followed by further purifification using the RNAeasy Mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Samples were processed and labeled with the Affymetrix 3’ IVT Express Kit with biotin according to the standard Affymetrix protocol from 5ug of total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized to Affymetrix Mouse Genome 430A_2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
8542 Dox.cel
|
| Data processing |
Analyses were performed using BRB-ArrayTools developed by Dr. Richard Simon and BRB-ArrayTools Development Team.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Dec 05, 2011 |
| Contact name |
Matthew J Simmons |
| Organization name |
UMASS Medical School
|
| Department |
Cancer Biology
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE34146 |
Transcriptional profiling of MMTV-tTA/TOP-ICN1 tumor derived cell lines comparing untreated (ICN1-On) cells to 24-hour doxcycline treated (ICN1-Off) samples |
|
