|
| Status |
Public on Feb 27, 2012 |
| Title |
4860956003_K |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Spleen
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human CD4 from NOD/SCID IL2gc-/- (NSG) reconstituted with human CD34+ at birth
treatment: Teplizumab in vitro
|
| Treatment protocol |
In vivo humanized mice were treated with teplizumab and sacrificed after 18 hours. In vitro splenocytes where isolated from humanized and treated with either teplizumab or hIg and then havested after 18 hours
|
| Growth protocol |
For in vitro treatment cells where grown in AIM-V serum free medium (GIBCO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Human CD4 cells from spleen were purified by positive selection on magnetic-activated Dynabeads (Invitrogen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Feb 27, 2012 |
| Contact name |
Frank Waldron-Lynch |
| Organization name |
Yale University Medical School
|
| Department |
Human Translational Immunology
|
| Street address |
300 George Street
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE34163 |
Microarray analysis of the migrating human CD4+ T cell population from the spleen of humanized mice in response to treatment with Teplizumab or hIg |
|
