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Sample GSM8465987 Query DataSets for GSM8465987
Status Public on Sep 04, 2024
Title kidney biopsy [GSM3524295 re-analysis]
Sample type RNA
 
Source name kidney biopsy
Organism Homo sapiens
Characteristics rej7aaclust (nr, minor, tcmr1, tcmr2, eabmr, fabmr, labmr): TCMR1
tissue: kidney biopsy
Extracted molecule total RNA
Extraction protocol All human heart biopsies taken for clinical indication during the period specified above were included. Tissue was immediately placed in RNA later.
Tissue was homogenization in 0.5ml of Trizol reagent (Invitrogen, Carlsbad, CA) and total RNA was extracted and purified using the Rneasy Micro Kit (Quiagen, Ontario, Canada) (average yield 8µg/core, (RNA integrity number >7).
Label biotin
Label protocol Total RNA (0.5µg) was labeled using GeneChip 3` IVT Express Labeling kit. Quality of labeled aRNA was assessed on an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA) before hybridization to GeneChip® PrimeView™ Human Gene Expression Array   
 
Hybridization protocol (Affymetrix, Santa Clara, CA).  Amplified RNA (10ug) was hybridized for 16 hr at 45C on  GeneChip® PrimeView™ Human Gene Expression Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Gene Array Scanner 3000 7G (Affymetrix) and processed with GeneChip® Command Console® Software (AGCC)(Affymetrix).
Data processing Microarray data files were preprocessed using robust multi-chip averaging (RMA) implemented in Bioconductor.
 
Submission date Aug 18, 2024
Last update date Sep 04, 2024
Contact name Jessica Chang
E-mail(s) jjchang@ualberta.ca
Organization name University of Alberta
Department Medicine
Lab ATAGC
Street address 250 Heritage Medical Research Centre
City Edmonton
ZIP/Postal code T6G 2S2
Country Canada
 
Platform ID GPL13667
Series (1)
GSE275126 Subthreshold rejection activity in many kidney transplants currently classified as having no rejection.
Relations
Reanalysis of GSM3524295

Supplementary file Size Download File type/resource
GSM8465987_GSM3524295.CEL.gz 1.9 Mb (ftp)(http) CEL

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