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Sample GSM848745 Query DataSets for GSM848745
Status Public on Dec 14, 2011
Title Day 70 CPD Ethanol, Biological Rep2
Sample type RNA
Source name Day 70 CPD Ethanol
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: Male
tissue: Whole Brain (without olfactory bulb)
treament: Fetal alcohol exposed
model: CPD
# samples pooled: 2
Treatment protocol Day 70 male mice were subjected to CO2 asphyxiation followed by cervical disolaction. Whole brain tissue was dissected in under five minutes and flash frozen liquid nitrogen.
Growth protocol C57BL/6J mice were prenatally exposed to ethanol either via Trimister 1,2, or 3, injections or continous preference drinking (Labelled T1, T2, T3, or CPD, respectively).
Extracted molecule total RNA
Extraction protocol TRIzol according to manufacturers protocol
Label Biotin
Label protocol One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
Hybridization protocol Samples were then hybridized to Affymetrix miRNA 2.0 arrays for 16 hours at 45°C
Scan protocol Probe level (.CEL file) data was generated using Affymetrix Command Console v1.1.
Description miRNA Expression data from Day 70 males exposed prenatally to alcohol via mothers given free access to 10% ethanol and water as source of liquid from gestation day 0 (G0) to postnatal day 10 (P10).
Data processing Probes were summarized to gene level data in Partek Genomics Suite v6.6 (Partek, St. Louis, MO, USA) using the RMA algorithm. Partek software was used to determine ANOVA p-values and fold changes.
Submission date Dec 13, 2011
Last update date Dec 14, 2011
Contact name Benjamin I Laufer
Organization name Genentech
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
Platform ID GPL14613
Series (1)
GSE34413 Persistent Alterations in the imprinted miRNA and snoRNA populations in mouse models of fetal alcohol spectrum disorders (FASD)

Data table header descriptions
VALUE log2 RMA signal

Data table
1367452_st 3.70862
1367453_st 3.34005
1367454_st 3.64535
1367455_st 3.84032
1367456_st 3.62487
1367457_st 2.78186
1367458_st 2.79534
1367459_st 3.87774
1367460_st 2.48345
1367461_st 3.74142
1367462_st 2.94296
1367463_st 4.00972
1367464_st 3.32177
1367465_st 3.1467
1367466_st 2.58283
1367467_st 3.72731
1367468_st 3.60462
1367469_st 2.69971
1367470_st 2.86655
1367471_st 2.69814

Total number of rows: 20706

Table truncated, full table size 498 Kbytes.

Supplementary file Size Download File type/resource
GSM848745_M0017.CEL.gz 606.2 Kb (ftp)(http) CEL
Processed data included within Sample table

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