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Sample GSM848754 Query DataSets for GSM848754
Status Public on Dec 14, 2011
Title Day 70 T2 Ethanol, Biological Rep1
Sample type RNA
 
Source name Day 70 T2 Ethanol
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: Male
tissue: Whole Brain (with olfactory bulb)
treament: Fetal alcohol exposed
model: T2
# samples pooled: 3
Treatment protocol Day 70 male mice were subjected to CO2 asphyxiation followed by cervical disolaction. Whole brain tissue was dissected in under five minutes and flash frozen liquid nitrogen.
Growth protocol C57BL/6J mice were prenatally exposed to ethanol either via Trimister 1,2, or 3, injections or continous preference drinking (Labelled T1, T2, T3, or CPD, respectively).
Extracted molecule total RNA
Extraction protocol TRIzol according to manufacturers protocol
Label Biotin
Label protocol One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
 
Hybridization protocol Samples were then hybridized to Affymetrix miRNA 2.0 arrays for 16 hours at 45°C
Scan protocol Probe level (.CEL file) data was generated using Affymetrix Command Console v1.1.
Description miRNA expression data from day 70 males exposed prenatally to alcohol during Trimester 2 (GD 14 and 16) via maternal injections of 20% ethanol in 0.15M saline with a dosage of 2.5g/kg. Saline was administered twice separated by two hours.
Data processing Probes were summarized to gene level data in Partek Genomics Suite v6.6 (Partek, St. Louis, MO, USA) using the RMA algorithm. Partek software was used to determine ANOVA p-values and fold changes.
 
Submission date Dec 13, 2011
Last update date Dec 14, 2011
Contact name Benjamin I Laufer
Organization name Genentech
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platform ID GPL14613
Series (1)
GSE34413 Persistent Alterations in the imprinted miRNA and snoRNA populations in mouse models of fetal alcohol spectrum disorders (FASD)

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
1367452_st 2.73802
1367453_st 3.22961
1367454_st 3.53577
1367455_st 3.15959
1367456_st 2.84431
1367457_st 3.23029
1367458_st 2.96312
1367459_st 3.63281
1367460_st 2.42889
1367461_st 3.63873
1367462_st 2.67638
1367463_st 2.81922
1367464_st 3.41244
1367465_st 2.82895
1367466_st 3.5997
1367467_st 3.09848
1367468_st 3.84114
1367469_st 2.88494
1367470_st 2.92862
1367471_st 3.04675

Total number of rows: 20706

Table truncated, full table size 498 Kbytes.




Supplementary file Size Download File type/resource
GSM848754_M0026.CEL.gz 836.1 Kb (ftp)(http) CEL
Processed data included within Sample table

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