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Sample GSM848758 Query DataSets for GSM848758
Status Public on Dec 14, 2011
Title Day 70 T1 Ethanol, Biological Rep1
Sample type RNA
 
Source name Day 70 T1 Ethanol
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: Male
tissue: Whole Brain (with olfactory bulb)
treament: Fetal alcohol exposed
model: T1
# samples pooled: 3
Treatment protocol Day 70 male mice were subjected to CO2 asphyxiation followed by cervical disolaction. Whole brain tissue was dissected in under five minutes and flash frozen liquid nitrogen.
Growth protocol C57BL/6J mice were prenatally exposed to ethanol either via Trimister 1,2, or 3, injections or continous preference drinking (Labelled T1, T2, T3, or CPD, respectively).
Extracted molecule total RNA
Extraction protocol TRIzol according to manufacturers protocol
Label Biotin
Label protocol One microgram of total RNA was labeled using the Flash Tag Biotin HSR kit (Genisphere)
 
Hybridization protocol Samples were then hybridized to Affymetrix miRNA 2.0 arrays for 16 hours at 45°C
Scan protocol Probe level (.CEL file) data was generated using Affymetrix Command Console v1.1.
Description miRNA expression data from day 70 males exposed prenatally to alcohol during Trimester 1 (GD 8) via maternal injections of 20% ethanol in 0.15M saline with a dosage of 2.5g/kg. Ethanol was administered twice separated by two hours.
Data processing Probes were summarized to gene level data in Partek Genomics Suite v6.6 (Partek, St. Louis, MO, USA) using the RMA algorithm. Partek software was used to determine ANOVA p-values and fold changes.
 
Submission date Dec 13, 2011
Last update date Dec 14, 2011
Contact name Benjamin I Laufer
Organization name Genentech
Street address 1 DNA Way
City South San Francisco
State/province California
ZIP/Postal code 94080
Country USA
 
Platform ID GPL14613
Series (1)
GSE34413 Persistent Alterations in the imprinted miRNA and snoRNA populations in mouse models of fetal alcohol spectrum disorders (FASD)

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
1367452_st 2.58672
1367453_st 3.24976
1367454_st 3.12411
1367455_st 3.88707
1367456_st 3.02322
1367457_st 2.70916
1367458_st 3.10141
1367459_st 3.4299
1367460_st 2.75437
1367461_st 3.28996
1367462_st 3.28286
1367463_st 3.17733
1367464_st 3.70164
1367465_st 2.53402
1367466_st 3.2487
1367467_st 3.22499
1367468_st 4.1712
1367469_st 2.86979
1367470_st 2.84828
1367471_st 3.11913

Total number of rows: 20706

Table truncated, full table size 498 Kbytes.




Supplementary file Size Download File type/resource
GSM848758_M0030.CEL.gz 850.6 Kb (ftp)(http) CEL
Processed data included within Sample table

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