GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM848935 Query DataSets for GSM848935
Status Public on Oct 10, 2012
Title rhesus testis
Sample type SRA
Source name testis
Organism Macaca mulatta
Characteristics tissue: testis
gender: male
library type: strand-specific, dUTP protocal
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from five rhesus monkey tissues using the Trizol method and analyzed by an Agilent 2100 bio-analyzer (Agilent Technologies). Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Data processing testis.bam; genome build: rheMac2
testis.bed; genome build: rheMac2
raw reads were aligned by Tophat v1.2.0, detailed parameters can be in header information of *.bam file. *.bam files and *.bed filed were used for the analysis of transcript expression and transcript structure.
Submission date Dec 13, 2011
Last update date May 15, 2019
Contact name Jia-Yu Chen
Organization name Peking University
Department Institute of Molecular Medicine
Lab Lab of Genomic Medicine
Street address No.5 Yiheyuan Road Haidian District
City Beijing
ZIP/Postal code 100871
Country China
Platform ID GPL14954
Series (1)
GSE34426 Transcriptome study in rhesus monkey
SRA SRX111328
BioSample SAMN00765446

Supplementary file Size Download File type/resource
GSM848935_testis.bam 4.6 Gb (ftp)(http) BAM
GSM848935_testis.bed.gz 5.4 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap