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Sample GSM848935 Query DataSets for GSM848935
Status Public on Oct 10, 2012
Title rhesus testis
Sample type SRA
 
Source name testis
Organism Macaca mulatta
Characteristics tissue: testis
gender: male
library type: strand-specific, dUTP protocal
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from five rhesus monkey tissues using the Trizol method and analyzed by an Agilent 2100 bio-analyzer (Agilent Technologies). Poly (A)-positive RNA was purified with the Dynabeads mRNA purification kit (Invitrogen) from 5μg of total RNA with high quality (RNA integrity number >7.5), following the manufacturer's instructions. A strand-specific RNA-Seq library preparation on the basis of the incorporation of deoxy-UTP was performed.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Data processing testis.bam; genome build: rheMac2
testis.bed; genome build: rheMac2
raw reads were aligned by Tophat v1.2.0, detailed parameters can be in header information of *.bam file. *.bam files and *.bed filed were used for the analysis of transcript expression and transcript structure.
 
Submission date Dec 13, 2011
Last update date May 15, 2019
Contact name Jia-Yu Chen
E-mail(s) jiayujiayu2008@163.com
Organization name Peking University
Department Institute of Molecular Medicine
Lab Lab of Genomic Medicine
Street address No.5 Yiheyuan Road Haidian District
City Beijing
ZIP/Postal code 100871
Country China
 
Platform ID GPL14954
Series (1)
GSE34426 Transcriptome study in rhesus monkey
Relations
SRA SRX111328
BioSample SAMN00765446

Supplementary file Size Download File type/resource
GSM848935_testis.bam 4.6 Gb (ftp)(http) BAM
GSM848935_testis.bed.gz 5.4 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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