|
| Status |
Public on Jun 24, 2012 |
| Title |
G9 P2_AG1478 B |
| Sample type |
RNA |
| |
|
| Source name |
primary human keratinocytes treated with AG1478
|
| Organism |
Homo sapiens |
| Characteristics |
cell: primary human keratinocytes
|
| Treatment protocol |
vehicle (0.2% DMSO), the EGFR inhibitor AG1478 (10 uM), recombinant human BMP2/7 heterodimer (200 ng/ml) or a combination of AG1478+BMP2/7 (10 uM and 200 ng/ml, respectively) for 48 hours.
|
| Growth protocol |
Primary human keratinocytes (line Lka, passage 2) were cultured in Keratinocyte Serum Free Medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. A DNase I digestion was included. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4285752011_F
AG1478 treated replicate 2
|
| Data processing |
The bead-level data were preprocessed using BASH (Cairns et al. (2008) Bioinformatics 24(24):2921-2), a function from the beadarray package (Dunning et al (2007) Bioinformatics 23(16):2183-4) in Bioconductor, and also log base 2 transformed and quantile normalised using GeneSpring GX software.
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
Sep 14, 2015 |
| Contact name |
Chandra Chilamakuri |
| E-mail(s) |
datasubmissions@cruk.cam.ac.uk
|
| Organization name |
Cancer Research UK Cambridge Institute
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6884 |
| Series (2) |
| GSE34557 |
Diverse epigenetic strategies interact to control epidermal differentiation [Illumina bead array] |
| GSE34558 |
Diverse epigenetic strategies interact to control epidermal differentiation |
|
