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Sample GSM8514713 Query DataSets for GSM8514713
Status Public on Sep 25, 2024
Title medium cultured with hWJ-MSCs_A alone
Sample type protein
 
Source name Wharton’s jelly-derived human mesenchymal stem cell
Organism Homo sapiens
Characteristics treatment: medium cultured with hWJ-MSCs_A alone
Treatment protocol We collected the conditioned media from the following groups to perform the array: C2C12 alone, hWJ-MSCs alone (A, B, C), hPL-MSCs alone (A, B, C), and C2C12 cells co-cultured with hWJ-MSCs (A, B, C), or hPL-MSCs (A, B, C).
Growth protocol hWJ-MSCs and hPL-MSCs were maintained in MEM-a supplemented with 10% fetal bovine serum (FBS) and 0.5% gentamicin in humidifed atmosphere with 5% CO2 at 37℃. C2C12 were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 0.5% gentamicin in humidifed atmosphere with 5% CO2 at 37℃.
Extracted molecule protein
Extraction protocol The secreted proteins in the conditioned media collected from hMSCs alone culture, C2C12 alone culture, and co-culture of hMSCs and C2C12 cells
Label biotin
Label protocol Sample was labeled according to the protocol in the kit manual from Raybiotech.
 
Hybridization protocol Drying the glass slide, Blocking and Incubations. Biotin labeled extract was hybridized with antibody-spotted slide for 2hr at room temperature. After hybridization, slide was washed with 1X wash buffer I and 1X wash buffer II and de-ionized water. Detection Buffer including Cy3-Streptavidin was treated for 2hr at room temperature in dark. And slide was washed with 1X wash buffer I, II and DW.
Scan protocol Scanned on an GenePix 4100 scanner.
Data processing GenePix Software (v 6.1) was used for data extraction.
 
Submission date Sep 13, 2024
Last update date Sep 25, 2024
Contact name Sun Jeong Kim
E-mail(s) xhxhfn78@gmail.com
Phone 82262058050
Organization name ENCell Co. Ltd.
Street address 701, Yeongdong-daero, Gangnam-gu
City Seoul
ZIP/Postal code 06072
Country South Korea
 
Platform ID GPL34899
Series (1)
GSE277126 Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α

Data table header descriptions
ID_REF
VALUE normalized signal intensity(Positive control signals normalization)

Data table
ID_REF VALUE
1 202.5306231
2 24777.72997
3 194.9635449
4 235.9147918
5 289.7745838
6 462.4820163
7 310.6953295
8 346.3051094
9 286.2136059
10 386.3661118
11 448.2381044
12 325.829486
13 625.8418816
14 301.3477623
15 162.0244985
16 297.3416621
17 238.5855253
18 101.0427504
19 309.3599628
20 185.1708554

Total number of rows: 507

Table truncated, full table size 7 Kbytes.




Supplementary file Size Download File type/resource
GSM8514713_hWJ-A.gpr.gz 44.3 Kb (ftp)(http) GPR

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