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Sample GSM852561 Query DataSets for GSM852561
Status Public on Mar 27, 2013
Title murine un-diseased arterial tissue on 3 month high fat diet Replicate 2, b
Sample type RNA
 
Source name murine un-diseased arterial tissue on 3 month high fat diet
Organism Mus musculus
Characteristics strain: apoe-/-
age: 6-8 weeks
Sex: Female
tissue: artery
Treatment protocol mice were fed on 3 month high fat diet
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol RecoverAll total nucleic acid isolation kit (Applied Biosystems) see http://www3.appliedbiosystems.com/cms/groups/mcb_support/documents/generaldocuments/cms_056616.pdf
Label FAM
Label protocol N/A
 
Hybridization protocol N/A
Scan protocol N/A
Description Biological replicate 2 of 4. un-diseased arterial tissue on 3 month high fat diet
Data processing Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics. http://www.integromics.com/StatMiner.php]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202, snoRNA135, snoRNA429 and MammU6 for Card A and B) according to the Genorm algorithm.
 
Submission date Dec 22, 2011
Last update date Mar 27, 2013
Contact name Andreas Schober
E-mail(s) aschober@med.lmu.de
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
 
Platform ID GPL15053
Series (2)
GSE34646 MiRNA expression profile in murine un-diseased arterial tissue on 3 month high fat diet vs 10 month high fat diet
GSE34648 MiRNA expression profile in murine

Data table header descriptions
ID_REF
VALUE [delta] CT = CT,sample - CT,endogenous control.

Data table
ID_REF VALUE
ath-miR159a-4373390 14.5365
mmu-let-7a*-4395608 2.965
mmu-let-7a-4373169 10.372
mmu-let-7b*-4395515 3.314
mmu-let-7b-4373168 7.481
mmu-let-7c-1*-4395609 11.128
mmu-let-7c-4373167 2.996
mmu-let-7d*-4378108 5.746
mmu-let-7d-4395394 5.613
mmu-let-7e-4395517 3.491
mmu-let-7f*-4395528 6.399
mmu-let-7f-4373164 9.102
mmu-let-7g*-4395622 7.51
mmu-let-7g-4395393 10.579
mmu-let-7i*-4395283 6.894
mmu-let-7i-4395332 13.176
mmu-miR-100-4373160 9.657
mmu-miR-101a*-4395637 5.081
mmu-miR-101a-4395364 11.254
mmu-miR-101b-4395661 22.12

Total number of rows: 520

Table truncated, full table size 13 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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