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Sample GSM857378 Query DataSets for GSM857378
Status Public on Jan 07, 2012
Title Intestine_KO_day8_3 [exon-level]
Sample type RNA
 
Source name small intestine (jejunum)
Organism Mus musculus
Characteristics strain: C57B/6
genotype/variation: Dicer KO
age: 8 weeks
Treatment protocol Tamoxifen gavage to induce conditional knockout
Growth protocol Conditional knockout of Dicer exon 21 & 22 in C57B/6 mice of 8 weeks of age
Extracted molecule total RNA
Extraction protocol miRNeasy Mini (Qiagen)
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the Johns Hopkins Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description THua-DIC-Cre_TA_1014-3a-mmExon1_(MoEx-1_0-st-v1).CEL
Data processing affymetrix-algorithm-name = rma-exon-full : dabg
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.1.2800.19935
program-company = Affymetrix
The statistical analysis was performed using GeneSpring GX 10.
 
Submission date Jan 06, 2012
Last update date Jan 07, 2012
Contact name Tai-Chung Huang
E-mail(s) huang@jhmi.edu
Phone 410-502-6665
Organization name Johns Hopkins University School of Medicine
Department Institute of Genetic Medicine
Lab Akhilesh Pandey
Street address 733 N Broadway BRB R560
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL6193
Series (2)
GSE34905 Differential gene expression caused by Dicer knockout in small intestine
GSE34910 Differential expression caused by Dicer knockout in small intestine
Relations
Alternative to GSM857384 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE log2 rma
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
4304920 3.16301 0.111165
4304921 3.35563 0.341535
4304922 9.0771 1.56561e-08
4304923 6.82178 4.58604e-05
4304925 6.97206 7.19529e-06
4304927 6.38253 0.0016444
4304928 10.0525 2.98754e-08
4304929 11.0293 5.87294e-08
4304930 5.9739 0.000445282
4304931 5.13796 0.0050668
4304932 7.81997 9.30314e-07
4304934 4.88645 0.035018
4304935 5.04797 0.00711219
4304937 5.86341 0.00158897
4304938 8.27298 5.95528e-08
4304939 3.50366 0.0673736
4304940 6.20989 0.00716605
4304941 5.74418 0.0246675
4304943 7.05849 3.11021e-05
4304944 5.22721 0.976999

Total number of rows: 1180331

Table truncated, full table size 30754 Kbytes.




Supplementary file Size Download File type/resource
GSM857378_THua-DIC-Cre_TA_1014-3a-mmExon1_MoEx-1_0-st-v1_.rma-exon-full-dabg.chp.gz 11.1 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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