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Sample GSM857380 Query DataSets for GSM857380
Status Public on Jan 07, 2012
Title Intestine_ctrl_day8_2 [exon-level]
Sample type RNA
 
Source name small intestine (jejunum)
Organism Mus musculus
Characteristics strain: C57B/6
genotype/variation: wildtype CTRL
age: 8 weeks
Treatment protocol Tamoxifen gavage to induce conditional knockout
Growth protocol Conditional knockout of Dicer exon 21 & 22 in C57B/6 mice of 8 weeks of age
Extracted molecule total RNA
Extraction protocol miRNeasy Mini (Qiagen)
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the Johns Hopkins Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description THua-DIC-Cre_TA_ctrl-2a-mmExon1_(MoEx-1_0-st-v1).CEL
Data processing affymetrix-algorithm-name = rma-exon-full : dabg
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.1.2800.19935
program-company = Affymetrix
The statistical analysis was performed using GeneSpring GX 10.
 
Submission date Jan 06, 2012
Last update date Jan 07, 2012
Contact name Tai-Chung Huang
E-mail(s) huang@jhmi.edu
Phone 410-502-6665
Organization name Johns Hopkins University School of Medicine
Department Institute of Genetic Medicine
Lab Akhilesh Pandey
Street address 733 N Broadway BRB R560
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
 
Platform ID GPL6193
Series (2)
GSE34905 Differential gene expression caused by Dicer knockout in small intestine
GSE34910 Differential expression caused by Dicer knockout in small intestine
Relations
Alternative to GSM857386 (gene-level analysis)

Data table header descriptions
ID_REF
VALUE log2 rma
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
4304920 2.85037 0.511553
4304921 3.35563 0.437338
4304922 8.89161 1.40883e-07
4304923 5.71127 0.0230106
4304925 7.00262 0.000100022
4304927 6.56825 0.0412467
4304928 10.4955 4.68706e-09
4304929 11.1217 7.2914e-08
4304930 5.85348 0.0016427
4304931 7.25646 4.62974e-05
4304932 6.89701 0.000230693
4304934 5.22602 0.0213066
4304935 4.94481 0.080401
4304937 5.58146 0.0380631
4304938 7.15643 1.40841e-05
4304939 3.27769 0.0685628
4304940 5.756 0.0436171
4304941 6.06544 0.0137639
4304943 5.60338 0.00120161
4304944 5.78072 0.873928

Total number of rows: 1180331

Table truncated, full table size 30491 Kbytes.




Supplementary file Size Download File type/resource
GSM857380_THua-DIC-Cre_TA_ctrl-2a-mmExon1_MoEx-1_0-st-v1_.rma-exon-full-dabg.chp.gz 11.1 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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