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Sample GSM857385 Query DataSets for GSM857385
Status Public on Jan 07, 2012
Title Intestine_ctrl_day8_1 [gene-level]
Sample type RNA
Source name small intestine (jejunum)
Organism Mus musculus
Characteristics strain: C57B/6
genotype/variation: wildtype CTRL
age: 8 weeks
Treatment protocol Tamoxifen gavage to induce conditional knockout
Growth protocol Conditional knockout of Dicer exon 21 & 22 in C57B/6 mice of 8 weeks of age
Extracted molecule total RNA
Extraction protocol miRNeasy Mini (Qiagen)
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
Hybridization protocol Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the Johns Hopkins Microarray Core Facility.
Scan protocol Array scanning was performed according to the manufacturer's instruction (Affymetrix)
Description THua-DIC-Cre_TA_ctrl-1a-mmExon1_(MoEx-1_0-st-v1).CEL
Data processing affymetrix-algorithm-name = rma-gene-extended
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.1.2800.19935
program-company = Affymetrix
The statistical analysis was performed using GeneSpring GX 10.
Submission date Jan 06, 2012
Last update date Jan 07, 2012
Contact name Tai-Chung Huang
Phone 410-502-6665
Organization name Johns Hopkins University School of Medicine
Department Institute of Genetic Medicine
Lab Akhilesh Pandey
Street address 733 N Broadway BRB R560
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platform ID GPL6096
Series (2)
GSE34905 Differential gene expression caused by Dicer knockout in small intestine
GSE34910 Differential expression caused by Dicer knockout in small intestine
Alternative to GSM857379 (exon-level analysis)

Data table header descriptions
VALUE log2 rma

Data table
6815740 6.47791
6881276 5.03742
6946812 5.24693
6815739 7.40313
6946811 4.00256
6946810 5.53162
7012346 5.92462
6881273 3.84357
6946809 7.72449
6750200 5.43736
6881272 6.09848
6946808 8.50548
6750199 3.37524
6946807 7.15968
6750198 3.89965
6946806 8.16854
6815733 5.55326
6881269 7.22723
6946805 5.87921
7012341 3.50627

Total number of rows: 100843

Table truncated, full table size 1564 Kbytes.

Supplementary file Size Download File type/resource
GSM857385_THua-DIC-Cre_TA_ctrl-1a-mmExon1_MoEx-1_0-st-v1_.rma-gene-extended.chp.gz 630.3 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file
Processed data are available on Series record

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