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Sample GSM857415 Query DataSets for GSM857415
Status Public on Mar 26, 2012
Title P19_control_rep1_batch3
Sample type RNA
Source name control untransduced P19 cells
Organism Mus musculus
Characteristics cell type: undifferentiated P19 cells
Treatment protocol P19 cells and MEFs were infected with lentivirus in media containing polybrene (final concentration 8 µg /ml), and after 24 hours the cells were switched into fresh media. MEFs transduced with MyoD were switched to differentiation media (1% heat inactivated horse serum, 10 μg/mL insulin, 10 μg/mL transferrin) 48 hours after infection.
Growth protocol P19 cells were maintained in MEMα as previously described (Farah et al., 2000, PUBMED ID 10648228), and MEFs derived from Myod-/-/Myf5-/- mice were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Neuronally differentiated P19 cells were harvested 72 hours after infection. MEFs were harvested 36-40 hours after switching to differentiation media.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Affymetrix One-Cycle Eukaryotic Target Labeling Assay.
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description 3.P19.con.1
Data processing Probe intensities were corrected, normalized, and summarized by the simpleaffy package of Bioconductor (Wilson and Miller,2005). Differentially expressed genes were identified by the LIMMA package of Bioconductor (Wettenhall and Smyth, 2004).
Submission date Jan 06, 2012
Last update date Apr 05, 2017
Contact name Stephen Tapscott
Organization name Fred Hutch Cancer Research Center
Department Human Biology
Lab Tapscott
Street address 1100 Fairview N. Ave
City Seattle
State/province WASHINGTON
ZIP/Postal code 98103
Country USA
Platform ID GPL1261
Series (2)
GSE34907 Genetic and epigenetic determinants of neurogenesis and myogenesis [expression profiling]
GSE34908 Genetic and epigenetic determinants of neurogenesis and myogenesis

Data table header descriptions
VALUE RMA normalized

Data table
1415670_at 9.992249589
1415671_at 11.35012208
1415672_at 11.37372795
1415673_at 10.99296248
1415674_a_at 10.13965722
1415675_at 8.340145164
1415676_a_at 12.49304901
1415677_at 7.967401907
1415678_at 11.32571779
1415679_at 10.86247174
1415680_at 10.81521696
1415681_at 10.47787586
1415682_at 9.657577159
1415683_at 12.40511294
1415684_at 9.16739752
1415685_at 9.989342877
1415686_at 9.178329466
1415687_a_at 10.72128644
1415688_at 10.62250005
1415689_s_at 8.807584863

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM857415_MG430_2.0_01_P19_control-1.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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