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Sample GSM857420 Query DataSets for GSM857420
Status Public on Mar 26, 2012
Title P19_ND2_rep3_batch3
Sample type RNA
Source name P19 transduced with NeuroD2 lentivirus
Organism Mus musculus
Characteristics cell type: Neuronally differentiated P19 cells
Treatment protocol P19 cells and MEFs were infected with lentivirus in media containing polybrene (final concentration 8 µg /ml), and after 24 hours the cells were switched into fresh media. MEFs transduced with MyoD were switched to differentiation media (1% heat inactivated horse serum, 10 μg/mL insulin, 10 μg/mL transferrin) 48 hours after infection.
Growth protocol P19 cells were maintained in MEMα as previously described (Farah et al., 2000, PUBMED ID 10648228), and MEFs derived from Myod-/-/Myf5-/- mice were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Neuronally differentiated P19 cells were harvested 72 hours after infection. MEFs were harvested 36-40 hours after switching to differentiation media.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Affymetrix One-Cycle Eukaryotic Target Labeling Assay.
Hybridization protocol Standard Affymetrix hybridization protocol
Scan protocol Standard Affymetrix scanning protocol
Description 3.P19.ND.3
Data processing Probe intensities were corrected, normalized, and summarized by the simpleaffy package of Bioconductor (Wilson and Miller,2005). Differentially expressed genes were identified by the LIMMA package of Bioconductor (Wettenhall and Smyth, 2004).
Submission date Jan 06, 2012
Last update date Apr 05, 2017
Contact name Stephen Tapscott
Organization name Fred Hutch Cancer Research Center
Department Human Biology
Lab Tapscott
Street address 1100 Fairview N. Ave
City Seattle
State/province WASHINGTON
ZIP/Postal code 98103
Country USA
Platform ID GPL1261
Series (2)
GSE34907 Genetic and epigenetic determinants of neurogenesis and myogenesis [expression profiling]
GSE34908 Genetic and epigenetic determinants of neurogenesis and myogenesis

Data table header descriptions
VALUE RMA normalized

Data table
1415670_at 10.1884224
1415671_at 11.51992316
1415672_at 11.41774424
1415673_at 11.09550616
1415674_a_at 10.25873768
1415675_at 8.564055139
1415676_a_at 12.37533934
1415677_at 8.866524305
1415678_at 11.2848483
1415679_at 11.17164872
1415680_at 10.38774833
1415681_at 10.38645325
1415682_at 8.953840276
1415683_at 11.9673804
1415684_at 9.034841128
1415685_at 9.7763834
1415686_at 9.940446067
1415687_a_at 10.96125557
1415688_at 11.17458778
1415689_s_at 9.046834288

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.

Supplementary file Size Download File type/resource
GSM857420_MG430_2.0_06_P19_ND2i-3.CEL.gz 6.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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