|
Status |
Public on Nov 08, 2024 |
Title |
Atopic Dermatitis 002 |
Sample type |
RNA |
|
|
Source name |
skin biopsy
|
Organism |
Homo sapiens |
Characteristics |
disease: Atopic Dermatitis
|
Treatment protocol |
samples were snap-frozen without any treatment
|
Growth protocol |
NA
|
Extracted molecule |
total RNA |
Extraction protocol |
mRNA expression of 600 targets was analysed with the nCounter Human Immunology V2 panel including 20 customized probes (Nanostring Technologies, Seattle, WA, USA) on the nCounterĀ® platform (Nanostring Technologies) using 100 ng of RNA per skin sample.
|
Label |
NanoString indexing
|
Label protocol |
NanoString indexing
|
|
|
Hybridization protocol |
standard protocol
|
Scan protocol |
standard protocol
|
Description |
internal positive and negative controls were used AD_002
|
Data processing |
Data were normalized and log2 transformed using nSolverĀ® 4.0 (Nanostring Technologies). Basically, housekeeping probes to be used for normalization are selected based on the geNorm algorithm as implemented in the NormqPCR R library
|
|
|
Submission date |
Oct 24, 2024 |
Last update date |
Nov 08, 2024 |
Contact name |
JEREMY DI DOMIZIO |
E-mail(s) |
jeremy.di-domizio@chuv.ch
|
Phone |
+41213147669
|
Organization name |
CHUV
|
Street address |
Av Boveresses 155, 1066 Epalinges
|
City |
Lausanne |
State/province |
Vaud |
ZIP/Postal code |
1066 |
Country |
Switzerland |
|
|
Platform ID |
GPL19963 |
Series (1) |
GSE280220 |
Immune Modules to guide Diagnosis and Personalized Treatment of Inflammatory Skin Diseases |
|