|
| Status |
Public on Nov 08, 2024 |
| Title |
Atopic Dermatitis 014 |
| Sample type |
RNA |
| |
|
| Source name |
skin biopsy
|
| Organism |
Homo sapiens |
| Characteristics |
disease: Atopic Dermatitis
|
| Treatment protocol |
samples were snap-frozen without any treatment
|
| Growth protocol |
NA
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA expression of 600 targets was analysed with the nCounter Human Immunology V2 panel including 20 customized probes (Nanostring Technologies, Seattle, WA, USA) on the nCounter® platform (Nanostring Technologies) using 100 ng of RNA per skin sample.
|
| Label |
NanoString indexing
|
| Label protocol |
NanoString indexing
|
| |
|
| Hybridization protocol |
standard protocol
|
| Scan protocol |
standard protocol
|
| Description |
internal positive and negative controls were used
AD_014
|
| Data processing |
Data were normalized and log2 transformed using nSolver® 4.0 (Nanostring Technologies). Basically, housekeeping probes to be used for normalization are selected based on the geNorm algorithm as implemented in the NormqPCR R library
|
| |
|
| Submission date |
Oct 24, 2024 |
| Last update date |
Nov 08, 2024 |
| Contact name |
JEREMY DI DOMIZIO |
| E-mail(s) |
jeremy.di-domizio@chuv.ch
|
| Phone |
+41213147669
|
| Organization name |
CHUV
|
| Street address |
Av Boveresses 155, 1066 Epalinges
|
| City |
Lausanne |
| State/province |
Vaud |
| ZIP/Postal code |
1066 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL19963 |
| Series (1) |
| GSE280220 |
Immune Modules to guide Diagnosis and Personalized Treatment of Inflammatory Skin Diseases |
|
