|
| Status |
Public on Nov 11, 2024 |
| Title |
H82 DMSO biol rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
NCI-H82
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: small cell lung cancer
|
| Treatment protocol |
The cells were treated with 50 nM CDDO-me for 6 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16 h at 45 °C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human Clariom S type from Affymetrix. A Custom CDF Version 22 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
|
| |
|
| Submission date |
Oct 30, 2024 |
| Last update date |
Nov 11, 2024 |
| Contact name |
Nikolas Gunkel |
| E-mail(s) |
n.gunkel@dkfz-heidelberg.de
|
| Organization name |
German Cancer Research Center (DKFZ)
|
| Department |
Research Group Cancer Drug Development
|
| Street address |
Im Neuenheimer Feld 280
|
| City |
Heidelberg |
| State/province |
*Not Applicable |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23159 |
| Series (2) |
| GSE280642 |
Differential KEAP1/NRF2-mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy [array] |
| GSE280643 |
Differential KEAP1/NRF2-mediated signaling widens the therapeutic window of redox-targeting drugs in SCLC therapy |
|
