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Sample GSM862172 Query DataSets for GSM862172
Status Public on Feb 01, 2012
Title Cardiomyocytes derived from normal iPSC treated by Serca2a, rep1
Sample type RNA
 
Source name Normal iPSC treated by Serca2a
Organism Homo sapiens
Characteristics cell type: Cardiomyocytes (derived from normal iPSC)
genotype/variation: Normal
gene therapy: Serca2a
Treatment protocol iPSC were treated by Adenovirus-Serca2a
Growth protocol iPSCs were maitained with mTESR-1 medium on matrigel coated surfaces. iPSC-derived cardiomyocytes were maitained with DMEM plus 10% FBS in tissue culture dishes.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Hybridization were followed standard Affymetrix protocol in Affymetrix GeneChip Hybridization Oven 640
Scan protocol GeneChips were scanned using theAffymetrix GeneChip Scanner 3000 7G
Description Gene expression data from iPSC
Control-Serca2a-1
Control-Serca2a-1.CEL
Data processing The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method.
 
Submission date Jan 14, 2012
Last update date Feb 01, 2012
Contact name Leng Han
E-mail(s) hanleng0926@hotmail.com
Organization name Stanford University
Street address 265 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6244
Series (1)
GSE35108 Expression data from patient iPSC and iPSC-derived cardiomyocytes

Data table header descriptions
ID_REF
VALUE RMA log2 value

Data table
ID_REF VALUE
7892501 4.40668
7892502 5.4004
7892503 4.652677
7892504 9.515676
7892505 5.350044
7892506 5.855883
7892507 7.7833
7892508 6.547104
7892509 12.42194
7892510 5.225936
7892511 6.457189
7892512 8.915339
7892513 4.988232
7892514 11.95442
7892515 11.03179
7892516 5.184273
7892517 8.114618
7892518 4.399571
7892519 6.711983
7892520 10.42176

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM862172.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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