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Sample GSM862176 Query DataSets for GSM862176
Status Public on Feb 01, 2012
Title Cardiomyocytes derived from DCM iPSC treated by Serca2a, rep1
Sample type RNA
 
Source name DCM iPSC treated by Serca2a
Organism Homo sapiens
Characteristics cell type: Cardiomyocytes (derived from DCM iPSC)
genotype/variation: DCM
gene therapy: Serca2a
Treatment protocol iPSC were treated by Adenovirus-Serca2a
Growth protocol iPSCs were maitained with mTESR-1 medium on matrigel coated surfaces. iPSC-derived cardiomyocytes were maitained with DMEM plus 10% FBS in tissue culture dishes.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol
 
Hybridization protocol Hybridization were followed standard Affymetrix protocol in Affymetrix GeneChip Hybridization Oven 640
Scan protocol GeneChips were scanned using theAffymetrix GeneChip Scanner 3000 7G
Description Gene expression data from iPSC
DCM-Serca2a-1
DCM-Serca2a-1.CEL
Data processing The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method.
 
Submission date Jan 14, 2012
Last update date Feb 01, 2012
Contact name Leng Han
E-mail(s) hanleng0926@hotmail.com
Organization name Stanford University
Street address 265 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platform ID GPL6244
Series (1)
GSE35108 Expression data from patient iPSC and iPSC-derived cardiomyocytes

Data table header descriptions
ID_REF
VALUE RMA log2 value

Data table
ID_REF VALUE
7892501 5.447927
7892502 6.093373
7892503 4.062257
7892504 9.178521
7892505 4.228799
7892506 6.518661
7892507 7.281698
7892508 7.975312
7892509 12.73104
7892510 6.365911
7892511 5.939511
7892512 8.06691
7892513 4.695869
7892514 11.81532
7892515 10.89049
7892516 5.464032
7892517 7.684548
7892518 4.699284
7892519 6.464307
7892520 10.56154

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM862176.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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