|
| Status |
Public on Nov 28, 2023 |
| Title |
SSC_cultured_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
spermatogonial stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: spermatogonial stem cells
strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit according to the prescribed protocol provided with the kit. Quality control was performed with nanodrop-1000 and electrophoresis.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPre RNA Amplification kit(IL1791) for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio
|
| |
|
| Submission date |
Jan 17, 2012 |
| Last update date |
Nov 28, 2023 |
| Contact name |
Ji Wu |
| E-mail(s) |
jiwu@sjtu.edu.cn
|
| Organization name |
Shanghai Jiao Tong University
|
| Street address |
No.800, Dongchuan Road, Minhang District
|
| City |
Shanghai |
| ZIP/Postal code |
200240 |
| Country |
China |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE35130 |
Genomic profiling of spermatogonial stem cells differentiation into putative female stem cells |
|
