|
| Status |
Public on Dec 12, 2024 |
| Title |
embryos 12-16HPE control 1 |
| Sample type |
SRA |
| |
|
| Source name |
embryo
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: embryo
genotype: Sima 1/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using a Qiagen Rneasy kit. 2ug total RNA was used for library construction.
Illumina TruSeq Stranded mRNA HT sample prep
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
Library name: 12-16 control 1
Sima_embryo_FPKMs.csv
|
| Data processing |
Aligned to BDGP6.46 genome assembly from Ensembl and clipped using star/2.7.11a
Read assignment and count matrix creation was completed using featureCounts from the Bioconductor Rsubread library and the BDGP6.46.113 annotation from Ensembl
Normalization was completed using DESeq2
Assembly: BDGP6.46
Supplementary files format and content: csv file containing FPKMs for larval samples and csv file containing FPKMs for embryo samples
|
| |
|
| Submission date |
Dec 11, 2024 |
| Last update date |
Dec 12, 2024 |
| Contact name |
Jason M Tennessen |
| E-mail(s) |
jtenness@iu.edu
|
| Organization name |
Indiana University
|
| Street address |
1001 E 3rd St
|
| City |
Bloomington |
| State/province |
Indiana |
| ZIP/Postal code |
47405 |
| Country |
USA |
| |
|
| Platform ID |
GPL30203 |
| Series (1) |
| GSE284097 |
The Drosophila Hypoxia-inducible factor 1-alpha is required to establish the larval glycolytic program |
|
| Relations |
| BioSample |
SAMN45771126 |
| SRA |
SRX27051311 |
