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Sample GSM873429 Query DataSets for GSM873429
Status Public on Apr 11, 2013
Title Wild-type Tm-RNA-seq
Sample type SRA
Source name Mouse embronic fibroblast
Organism Mus musculus
Characteristics cell type: Mouse embryonic fibroblast
developmental stage: 11.5-12.5 days
strain: C57BL/6
genotype/variation: WT
treatment: Tunicamycin
Treatment protocol MEFs were treated with tunicamycin for 10hr. After treatment, chromatin immunoprecipitation or RNA isolation were performed.
Growth protocol Mouse embryonic fibroblast cells were rountinely subcultured in high-glucose DMEM supplemented with 10% FBS, Pen/Strep, NEAA (all invitorgen) on cell culture dishes.
Extracted molecule total RNA
Extraction protocol unknown
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer
Description WT Treatment
Data processing ERANGE was used to create .rds database files and then wig files with -raw option turned on.
ChIP-Seq peaks were identified using ERANG with default settings
rpkm files: mm9
Submission date Feb 09, 2012
Last update date May 15, 2019
Contact name Maureen A Sartor
Organization name University of Michigan
Department Department of Computational Medicine and Bioinformatics
Lab Sartor
Street address 2017 Palmer Commons Bldg., 100 Washtenaw Ave
City Ann Arbor
State/province MI
ZIP/Postal code 48109-2218
Country USA
Platform ID GPL9185
Series (1)
GSE35681 ChIP-seq and mRNA-seq experiment to find the direct target genes of ATF4 and CHOP
Reanalyzed by GSE80793
SRA SRX119355
BioSample SAMN00787460

Supplementary file Size Download File type/resource 238.6 Kb (ftp)(http) RPKM 238.3 Kb (ftp)(http) RPKM
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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