|
| Status |
Public on Mar 07, 2013 |
| Title |
Rec12_wt |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
FLAG ChIP DNA from rec12+-FLAG wild type cells harvested 5h after meiosis induction
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
cell type: rec12+-FLAG wild type cells harvested 5h after meiosis induction
fraction: ChIP
antibody: FLAG
|
| Growth protocol |
Cells in a pat1-114 rad50S rec12+-FLAG background were induced to enter meiosis by the haploid meiosis system, and harvested five hours after the induction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using following antibodies. Anti-H3K9ac and -H3K14ac antibodies were purchased from Millipore. Anti-H3K4me3, and -H3 antibodies were from Abcam. Anti-FLAG was from Sigma-Aldrich.
|
| Label |
biotin
|
| Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
| |
|
| Channel 2 |
| Source name |
Input DNA from rec12+-FLAG wild type cells harvested 5h after meiosis induction
|
| Organism |
Schizosaccharomyces pombe |
| Characteristics |
cell type: rec12+-FLAG wild type cells harvested 5h after meiosis induction
fraction: input
|
| Growth protocol |
Cells in a pat1-114 rad50S rec12+-FLAG background were induced to enter meiosis by the haploid meiosis system, and harvested five hours after the induction.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except for using following antibodies. Anti-H3K9ac and -H3K14ac antibodies were purchased from Millipore. Anti-H3K4me3, and -H3 antibodies were from Abcam. Anti-FLAG was from Sigma-Aldrich.
|
| Label |
biotin
|
| Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
| |
|
| |
| Hybridization protocol |
16 h at 45C using a hybridization oven 640 (affymetrix) as described in the Affymetrix Chromatin Immunoprecipitation Assay Protocol (P/N 702238)
|
| Scan protocol |
Fluidics station 400 protocol EukGE-WS2v5 (affymetrix) was performed to wash and stain arrays. The arrays were scanned on GeneChip Scanner 3000 7G (affymetrix).
|
| Description |
Rec12 / WCE, meiosis 5h, wild type cells
input CEL: WCE_2.CEL
|
| Data processing |
Data were quantile normalized and smoothed with Affymetrix Tiling Analysis Software
|
| |
|
| Submission date |
Feb 13, 2012 |
| Last update date |
Mar 07, 2013 |
| Contact name |
Shintaro YAMADA |
| E-mail(s) |
ss107140@mail.ecc.u-tokyo.ac.jp
|
| Organization name |
University of Tokyo
|
| Street address |
3-8-1 Komaba
|
| City |
Meguro-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
153-8902 |
| Country |
Japan |
| |
|
| Platform ID |
GPL7715 |
| Series (1) |
| GSE31648 |
Histone H3 lysine9 acetylation, rather than lysine4 trimethylation, marks meiotic recombination hotspots and promotes recombination initiation in fission yeast |
|
