|
| Status |
Public on Dec 01, 2012 |
| Title |
099 Migratory vs. non-migratory (MM/MNM) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
098 Control-Migratory (099 M5)
|
| Organism |
Homo sapiens |
| Characteristics |
msc type: 099 Control-Migratory (099 M5)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
2 µg of the respective total RNAs were each mixed with 2.5 fmol of each of 18 RNA oligonucleotides reverse complement to miRControl 3 probes and subsequently fluorescently labelled using a commercial kit (miRCuryTM LNA microRNA Array Power labeling kit, Exiqon) following the instructions of the manufacturer. The total RNA mix was hybridized in a dual colour approach to microarrays.
|
| |
|
| Channel 2 |
| Source name |
Human breast adipose tissue derived MSCs (B-ASCs)
|
| Organism |
Homo sapiens |
| Characteristics |
msc type: 099 non-migratory (MNM)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol following manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
2 µg of the respective total RNAs were each mixed with 2.5 fmol of each of 18 RNA oligonucleotides reverse complement to miRControl 3 probes and subsequently fluorescently labelled using a commercial kit (miRCuryTM LNA microRNA Array Power labeling kit, Exiqon) following the instructions of the manufacturer. The total RNA mix was hybridized in a dual colour approach to microarrays.
|
| |
|
| |
| Hybridization protocol |
Hy3 and Hy5 labeled (sample_ch1 and sample_ch2) RNA were combined and hybridized overnight to miRXplore TM Microarrays using the a-HybTM Hybridization Station (Miltenyi Biotec).
|
| Scan protocol |
Image capture and signal quantification of hybridized miRXplore (TM) microarrays were done with the Agilent’s Microarray Scanner System (G2505C, Agilent Technologies, Palo Alto, USA) and ImaGene software Version 8.0.1 (BioDiscovery, Los Angeles, CA, USA).
|
| Data processing |
Signal quantification was done using ImaGene software version 8.0.1 (BioDiscovery, Los Angeles, MA). Normalization was performed using the calibration oligos
|
| |
|
| Submission date |
Feb 17, 2012 |
| Last update date |
Dec 01, 2012 |
| Contact name |
Reza Izadpanah |
| E-mail(s) |
rizadpan@tulane.edu
|
| Organization name |
Tulane University
|
| Department |
Medicien
|
| Lab |
Applied Stem Cell
|
| Street address |
1430 Tulane Avenue
|
| City |
New Orleans |
| State/province |
Louisiana |
| ZIP/Postal code |
70112 |
| Country |
USA |
| |
|
| Platform ID |
GPL15234 |
| Series (1) |
| GSE35901 |
MicroRNA expression analysis of tumor tropic mesenchymal stem cells |
|
