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Sample GSM878551 Query DataSets for GSM878551
Status Public on Feb 23, 2012
Title TG mouse lung, biological rep2
Sample type RNA
 
Source name TG mouse lung
Organism Mus musculus
Characteristics tissue: mouse lung upper left lobe
strain: C57BL/6
genotype/variation: CC10-rtTA-IL13 transgenic (TG)
age: 6-10 weeks old mouse
Treatment protocol Mice were given doxycycline-treated (final concentration 2 mg/mL) water for 7 days. After 7 days, mice were euthanized and the left upper lobes of the lungs were removed for RNA extraction.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Following fragmentation, cRNA were hybridized for 16 hr at 45C on GeneChip Mouse miRNA Array. GeneChips were washed and stained according to standard Affymetrix protocol.
Scan protocol GeneChips were scanned according to standard protocol.
Description miRNA expression data from mouse lung upper left lobe
Data processing The raw expression data were first processed for background estimation and correction, normalization, and summarization using miRNAQCTools, an Affymetrix software specifically developed for the GeneChip miRNA arrays.
 
Submission date Feb 21, 2012
Last update date Feb 23, 2012
Contact name Aik T Ooi
Organization name UCLA
Department Pediatrics
Street address 650 Charles E Young Dr S, Factor 9-240
City Los Angeles
ZIP/Postal code CA
Country USA
 
Platform ID GPL8786
Series (2)
GSE35980 MicroRNA expression data from IL13-induced allergic airway inflammation of mice lungs
GSE37085 Expression and MeDIP microarray data from IL13-induced allergic airway inflammation of mice lungs

Data table header descriptions
ID_REF
VALUE Processed microarray expression data in log2.

Data table
ID_REF VALUE
hsa-miR-1826_st 12.60005
mml-miR-127_st 7.078467
rno-miR-146b_st 7.296182
ptr-miR-127_st 7.029526
hsa-miR-127-3p_st 6.912371
age-miR-127_st 6.829375
ppy-miR-127_st 6.924482
bta-miR-127_st 6.921511
mmu-miR-685_st 7.022548
lla-miR-127_st 6.515505
bta-miR-532_st 6.355712
rno-miR-324-5p_st 6.069165
rno-miR-324-3p_st 5.464394
hsa-miR-502-3p_st 5.235396
mmu-miR-379_st 5.528433
hsa-miR-146b-5p_st 5.407187
mml-miR-21_st 5.511312
age-miR-21_st 5.383136
mmu-miR-699_st 5.272412
mmu-miR-362-5p_st 5.290216

Total number of rows: 7625

Table truncated, full table size 183 Kbytes.




Supplementary file Size Download File type/resource
GSM878551.CEL.gz 145.3 Kb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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