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Sample GSM885547 Query DataSets for GSM885547
Status Public on Mar 06, 2012
Title Stretch 6 hours sample 6
Sample type RNA
 
Source name Stretch 6 hours
Organism Rattus norvegicus
Characteristics cell type: Primary alveolar epithelial cell
strain: Sprague Dawley
gender: male
weight: 200-300g
Treatment protocol Isolated type II cells from whole lung, grown for 5 days in MEM + 0.1x FBS until cells attain type I characteristics
Extracted molecule total RNA
Extraction protocol Quiagen miRNAeasy Kit per protocol; quality control with Agilent 2100 bioanalyzer
Label Hy5
Label protocol Adjust RNA and volume to 0.25ug to 1ug in 2ul of nuclease free water; add CIP buffer, enzyme, and spike-in RNA at 37C x 30min; quench 95C x 5min; snap cool on ice 2-15min; mix with Labeling buffer, enzyme, dye, and DMSO 16C x 1hr; quench 65C x 15min, cool to 4C; hybridization within 1 hour
 
Hybridization protocol Mix dyed samples with warmed hyb buffer; denature 95C x 3min; snap cool on ice 2-15min; centrifuge max 1min; apply sample to Agilent coverslip and slide, and assemble hyb apparatus; hyb 56C x 16hr rotation speed 4; wash 3x with buffers A, B, C 2min each, rinse with water, dry by centriguge 800rpm x 3min
Scan protocol Images acquired at 635 and 532 nm using GenePix 4000B, analyzed genePix pro 6.0.1.27
Description Each array was hybridized with each sample labeled with Hy5 against a Common Reference (labeled Hy3). Common Reference is an equal (same ng of RNA) mix of all sample groupings (Control, 1hr stretch, 6hr stretch) from each of the 12 individual samples pooled together.
Data processing The software was Partek Genomics Suite (version 6.4) where background subtracted intensities were extracted, any background intensity below 1 was changed to 1, and loess normalization was applied.
 
Submission date Mar 04, 2012
Last update date Mar 06, 2012
Contact name Nadir Yehya
E-mail(s) yehyan@email.chop.edu
Organization name University of Pennsylvania
Department Bioengineering
Street address 390 Towne Bldg, U Penn
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
 
Platform ID GPL7722
Series (1)
GSE36256 Differential expression of miRNA in stretched rat alveolar epithelial cells

Data table header descriptions
ID_REF
VALUE loess normalized intensity

Data table
ID_REF VALUE
-1 674
0 3
10138 600.5
10306 389
10482 1
10618 74
10898 300
10899 7001.5
10901 10
10902 8.5
10903 40
10904 4665
10905 2548
10906 1103
10907 15368.5
10914 2182.5
10919 185
10923 198.5
10925 31
10928 736.5

Total number of rows: 1279

Table truncated, full table size 12 Kbytes.




Supplementary file Size Download File type/resource
GSM885547_miRNA1149H_Ref__532_750__vs_8305__635_750__100308.gpr.gz 478.5 Kb (ftp)(http) GPR
Processed data included within Sample table

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