Columbia wild type (Col-0) with an active FRI allele introgressed from San Feliu-2 (Sf-2) grown in short days (9L:15D) for 30 days at 21°C, then shifted to long days (16L:8D).
Time point: 2 days
Alleles: FRIGIDA (FRI): Sf-2 FLOWERING LOCUS C (FLC): Col-0 Keywords = flowering