|
| Status |
Public on Sep 01, 2012 |
| Title |
2_nebulin_+/- [mRNA] |
| Sample type |
RNA |
| |
|
| Source name |
mouse HZ2 heterozygous
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: nebulin +/-
tissue: quadriceps
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from quadriceps using Trizol®-Reagent (Gibco brl). and Qiagen RNeasy Kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
quadriceps RNA of mouse HZ2 with one nebulin allele
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of Mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Mar 23, 2012 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14661 |
| Series (2) |
| GSE36743 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy [mRNA] |
| GSE36745 |
In vivo and in vitro investigations of heterozygous nebulin knock-out mice reveal similarities with mild human form of nemaline myopathy |
|
