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Status
Public on Dec 01, 2012
Title
UT-SCC-1A
Sample type
genomic
Source name
HNSCC
Organism
Homo sapiens
Characteristics
cell line: HNSCC cetuximab response: cetuximab resistant
Treatment protocol
Cells were seeded and allowed to attach for 24h, then treated with 30 nM cetuximab for 48h, supplemented with fresh medium and grown for another 7 days
Growth protocol
Cells were grown in DMEM supplemented with 10% FBS, 2 mM glutamine, 1% NE amino acids, and penicillin and streptomycin
Extracted molecule
genomic DNA
Extraction protocol
DNA was extracted with Maxwell 16 Cell purification kit
Label
biotin
Label protocol
DNA fragments were biotin labelled using terminal deoxynucleotidyl transferase
Hybridization protocol
Hybridized in GeneChip Hybrydization oven 16-18h, stained with R-phycoerythrin streptavidin and biotinylated goat-anti-streptavidin antibodies
Scan protocol
GeneChip scanner 3000 7G
Data processing
Genotyping console, CN5 algorithm
Submission date
Mar 26, 2012
Last update date
Dec 01, 2012
Contact name
Fredrik Jerhammar
E-mail(s)
fredrik.jerhammar@liu.se
Phone
+4613221500
Organization name
Linkoping University
Street address
-
City
Linkoping
ZIP/Postal code
58183
Country
Sweden
Platform ID
GPL6801
Series (1)
GSE36790
Cetuximab resistant or sensitive head and neck cancer samples
Data table header descriptions
ID_REF
VALUE
CN STATE
LOG2 RATIO
SMOOTH SIGNAL
LOH
ALLELE DIFFERENCE
Data table
ID_REF
VALUE
LOG2 RATIO
SMOOTH SIGNAL
LOH
ALLELE DIFFERENCE
CN_473963
3.000000
0.345972
2.615029
nan
nan
CN_473964
3.000000
-0.129647
2.615235
nan
nan
CN_473965
3.000000
0.026661
2.615277
nan
nan
CN_473981
3.000000
-0.086503
2.618372
nan
nan
CN_473982
3.000000
-0.026221
2.618420
nan
nan
CN_497981
3.000000
0.194448
2.646285
nan
nan
CN_502615
3.000000
0.310079
2.683631
nan
nan
CN_502613
3.000000
0.219880
2.683806
nan
nan
CN_502614
3.000000
0.660838
2.684730
nan
nan
CN_502616
3.000000
0.807727
2.684778
nan
nan
CN_502843
3.000000
0.156742
2.719299
nan
nan
CN_466171
3.000000
-0.010981
2.332301
nan
nan
CN_468414
3.000000
0.124228
2.331797
nan
nan
CN_468412
3.000000
0.996604
2.331684
nan
nan
CN_468413
3.000000
0.527137
2.331663
nan
nan
CN_470565
3.000000
0.251031
2.331054
nan
nan
CN_468424
2.000000
-0.583350
2.322777
nan
nan
CN_468425
2.000000
-0.454296
2.322697
nan
nan
CN_460512
2.000000
0.352987
2.618792
nan
nan
CN_460513
2.000000
0.079331
2.618791
nan
nan
Total number of rows: 1813441 Table truncated, full table size 93639 Kbytes .Supplementary file
Size
Download
File type/resource
GSM901092_UTSCC-1_GenomeWideSNP_6_.CEL.gz
29.2 Mb
(ftp) (http)
CEL
GSM901092_UTSCC-1_GenomeWideSNP_6_.CN5.CNCHP.gz
34.8 Mb
(ftp) (http)
CNCHP
Processed data included within Sample table
Processed data provided as supplementary file