|
| Status |
Public on Apr 03, 2012 |
| Title |
TOLEDO |
| Sample type |
genomic |
| |
|
| Source name |
B-NHL cell line
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: B-cell Non Hodgkin Lymphoma
cell line: TOLEDO
|
| Treatment protocol |
No special treatment was applied to the cells.
|
| Growth protocol |
Cells were grown according to the conditions indicated by ATCC or DMSZ for each cell line.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA from frozen cell pellets was isolated using the QUIAGEN Gentra Purigene Cell Kit, according to the manufacturer instructions.
|
| Label |
biotin
|
| Label protocol |
Genomic DNA preparation, labeling and hybridization were performed according to Affymetrix's recommended protocols (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Genomic DNA preparation, labeling and hybridization were performed according to Affymetrix's recommended protocols (Affymetrix, Santa Clara, CA, USA).Label used was biotin.
|
| Scan protocol |
Arrays were scanned with GeneChip GSC3000 7G Whole-Genome Association System (Affymetrix).
|
| Data processing |
Data were analyzed using Biodiscovery Nexus 6.0 software; The Value field is the log2 of the ratio of probe intensity (Pi) of the sample over the Reference intensity (Ri). Value = Log2(Pi/Ri). The Reference intensity has been calculated form hapmap sample arrays.
10 B-NHL cell lines. Affymetrix SNP 6.0 Arrays were used.
|
| |
|
| Submission date |
Mar 30, 2012 |
| Last update date |
Apr 03, 2012 |
| Contact name |
Claudio Scuoppo |
| E-mail(s) |
scuoppo@gmail.com
|
| Organization name |
Cold Spring Harbor
|
| Lab |
Scuoppo
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
- |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platform ID |
GPL6801 |
| Series (1) |
| GSE36967 |
Affymetrix SNP 6.0 profiles of 10 Human B-NHL lines |
|
