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Sample GSM908116 Query DataSets for GSM908116
Status Public on Mar 04, 2013
Title E176_01_Leptin_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_01_Leptin_3
treatment: Leptin
weeks of treatment: 3
subject: E176_01
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.678236032
10001_at 5.856940492
10002_at 2.745933372
10003_at 2.389258339
100048912_at 2.300219377
10004_at 3.657518145
10005_at 4.886122761
10006_at 7.488465724
10007_at 5.471891543
10009_at 2.125039207
1000_at 2.675743934
10010_at 6.030370433
100126791_at 3.534139811
100128124_at 2.942850481
100128640_at 3.428152396
100129128_at 2.073939542
100129250_at 6.205801982
100129271_at 3.534854505
100129482_at 5.067698203
100129973_at 2.633543806

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908116_Holly_01.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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