NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM908122 Query DataSets for GSM908122
Status Public on Mar 04, 2013
Title E176_05_Placebo_2_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_05_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_05
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 2.694438509
10001_at 4.063191834
10002_at 2.834727599
10003_at 3.398450449
100048912_at 2.796917029
10004_at 3.482090156
10005_at 5.817944562
10006_at 6.717589663
10007_at 4.009496772
10009_at 3.168474271
1000_at 2.673454923
10010_at 5.142352791
100126791_at 3.563893028
100128124_at 2.825560264
100128640_at 4.161729975
100129128_at 2.188719258
100129250_at 5.719201729
100129271_at 4.406159512
100129482_at 4.48927026
100129973_at 2.7938711

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908122_Holly_07.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap