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Sample GSM908123 Query DataSets for GSM908123
Status Public on Mar 04, 2013
Title E176_05_Placebo_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_05_Placebo_3
treatment: Placebo
weeks of treatment: 3
subject: E176_05
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 3.662499566
10001_at 3.872059486
10002_at 3.695352321
10003_at 3.035911159
100048912_at 3.193456523
10004_at 3.61700416
10005_at 5.452092779
10006_at 6.302484604
10007_at 4.261037819
10009_at 2.629142251
1000_at 3.270561922
10010_at 5.483670373
100126791_at 4.760715573
100128124_at 3.386264868
100128640_at 3.802284857
100129128_at 2.234094951
100129250_at 6.034878048
100129271_at 2.753602157
100129482_at 5.00004624
100129973_at 3.402226937

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908123_Holly_08.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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