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Sample GSM908128 Query DataSets for GSM908128
Status Public on Mar 04, 2013
Title E176_08_Placebo_2_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_08_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_08
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.696182634
10001_at 5.269959827
10002_at 2.568674869
10003_at 2.423165386
100048912_at 2.384090989
10004_at 3.752239134
10005_at 5.197024987
10006_at 7.532350143
10007_at 5.153787735
10009_at 2.541005287
1000_at 2.645711962
10010_at 6.228671689
100126791_at 3.50055921
100128124_at 2.568665348
100128640_at 3.429886926
100129128_at 2.242620861
100129250_at 6.187336487
100129271_at 2.871309605
100129482_at 4.797901261
100129973_at 2.443995306

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908128_Holly_13.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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