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Sample GSM908130 Query DataSets for GSM908130
Status Public on Mar 04, 2013
Title E176_09_Placebo_1_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_09_Placebo_1
treatment: Placebo
weeks of treatment: 1
subject: E176_09
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 2.685938743
10001_at 5.589843595
10002_at 2.372345685
10003_at 2.656243788
100048912_at 2.428788461
10004_at 3.285088698
10005_at 4.844259317
10006_at 7.463575247
10007_at 5.764019487
10009_at 2.355269246
1000_at 2.701161831
10010_at 6.312282884
100126791_at 3.3270523
100128124_at 2.6290327
100128640_at 2.5995644
100129128_at 2.312001081
100129250_at 5.749838661
100129271_at 2.622606409
100129482_at 4.835936515
100129973_at 2.369214152

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908130_Holly_15.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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