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Sample GSM908134 Query DataSets for GSM908134
Status Public on Mar 04, 2013
Title E176_11_Leptin_2_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_11_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_11
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.829239184
10001_at 5.577805262
10002_at 2.439347302
10003_at 2.470810428
100048912_at 2.442272006
10004_at 3.50856421
10005_at 5.095657409
10006_at 7.378534644
10007_at 5.782300659
10009_at 2.461685646
1000_at 2.737442656
10010_at 6.177547478
100126791_at 2.999101533
100128124_at 2.79843591
100128640_at 3.182144741
100129128_at 2.27177111
100129250_at 6.178277601
100129271_at 2.869333573
100129482_at 4.554518703
100129973_at 2.422465696

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908134_Holly_20.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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