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Sample GSM908137 Query DataSets for GSM908137
Status Public on Mar 04, 2013
Title E176_14_Leptin_2_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_14_Leptin_2
treatment: Leptin
weeks of treatment: 2
subject: E176_14
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 3.389073307
10001_at 4.854968823
10002_at 3.348264293
10003_at 4.757808926
100048912_at 2.419502524
10004_at 2.885914189
10005_at 3.807964959
10006_at 5.453842641
10007_at 3.176155099
10009_at 2.851510496
1000_at 3.185181492
10010_at 4.696549998
100126791_at 5.241842546
100128124_at 2.751148957
100128640_at 2.633251203
100129128_at 2.474646291
100129250_at 5.788591122
100129271_at 3.460181059
100129482_at 3.716974331
100129973_at 3.004587541

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908137_Holly_23.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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