NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM908139 Query DataSets for GSM908139
Status Public on Mar 04, 2013
Title E176_12_Placebo_2_A
Sample type RNA
 
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_12_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_12
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
 
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
 
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
E-mail(s) dario.greco@tuni.fi
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
 
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
ID_REF
VALUE log2 RMA normalized signal

Data table
ID_REF VALUE
10000_at 2.681452673
10001_at 5.417244405
10002_at 2.42274951
10003_at 2.538283836
100048912_at 2.256130109
10004_at 3.908902308
10005_at 5.272553808
10006_at 7.339297488
10007_at 5.92919695
10009_at 2.401730397
1000_at 2.655196268
10010_at 6.014368579
100126791_at 3.462347612
100128124_at 2.728384298
100128640_at 2.871322103
100129128_at 2.212131659
100129250_at 5.962622062
100129271_at 2.914874763
100129482_at 4.257340478
100129973_at 2.426332484

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.




Supplementary file Size Download File type/resource
GSM908139_Holly_25.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap