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Sample GSM908144 Query DataSets for GSM908144
Status Public on Mar 04, 2013
Title E176_17_Placebo_2_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_17_Placebo_2
treatment: Placebo
weeks of treatment: 2
subject: E176_17
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.772202129
10001_at 4.770029953
10002_at 2.834900474
10003_at 2.303152504
100048912_at 2.697717182
10004_at 3.499297425
10005_at 4.835177672
10006_at 7.276173656
10007_at 4.650972549
10009_at 2.795408819
1000_at 3.13840291
10010_at 5.84210298
100126791_at 3.353599242
100128124_at 2.681216667
100128640_at 3.364380477
100129128_at 2.370791358
100129250_at 6.090018711
100129271_at 3.396044754
100129482_at 3.793369454
100129973_at 2.308169861

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908144_Holly_30.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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