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Sample GSM908148 Query DataSets for GSM908148
Status Public on Mar 04, 2013
Title E176_15_Leptin_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_15_Leptin_3
treatment: Leptin
weeks of treatment: 3
subject: E176_15
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.746718076
10001_at 4.304194312
10002_at 3.065188491
10003_at 2.946341059
100048912_at 2.71398895
10004_at 3.682482342
10005_at 4.279699959
10006_at 6.518904003
10007_at 4.091103176
10009_at 2.649394011
1000_at 3.194247471
10010_at 5.082068561
100126791_at 6.016460243
100128124_at 3.118543092
100128640_at 3.596351232
100129128_at 2.258249062
100129250_at 5.876512174
100129271_at 3.73895852
100129482_at 4.502523105
100129973_at 3.338187404

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908148_Holly_34.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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