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Sample GSM908151 Query DataSets for GSM908151
Status Public on Mar 04, 2013
Title E176_29_Placebo_1_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_29_Placebo_1
treatment: Placebo
weeks of treatment: 1
subject: E176_29
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.500455604
10001_at 4.600177696
10002_at 3.271484598
10003_at 2.821132086
100048912_at 2.570590866
10004_at 3.57199364
10005_at 5.188522538
10006_at 6.851659892
10007_at 4.272002953
10009_at 3.224754646
1000_at 2.854069676
10010_at 5.925631253
100126791_at 3.325461896
100128124_at 3.064971195
100128640_at 3.620415134
100129128_at 2.239964251
100129250_at 5.94417712
100129271_at 2.859358756
100129482_at 4.23079549
100129973_at 3.506809318

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908151_Holly_37.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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