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Sample GSM908153 Query DataSets for GSM908153
Status Public on Mar 04, 2013
Title E176_27_Leptin_3_A
Sample type RNA
Source name PBMC
Organism Homo sapiens
Characteristics sample: E176_27_Leptin_3
treatment: Leptin
weeks of treatment: 3
subject: E176_27
Treatment protocol Human PBMCs were isolated by stratifying of whole blood on Ficoll-Paque PREMIUM (GE Healthcare; Pscataway, NJ). Subjects were randomized in a 1:1 fashion to receive either metreleptin or placebo, for a period of 36 weeks in the form of a self-administered daily subcutaneous injection.
Extracted molecule total RNA
Extraction protocol RNA was isolated from PBMCs using the manufacturer’s protocol for Trizol Reagent (Invitrogen, Grand Island, NY) and further purified using RNAeasy spin columns (Qiagen, Valencia, CA). RNA concentration and purity were determined from 260nm/280 nm absorbances. RNA integrity was determined using the Agilent 2100 BioChip (Agilent Technologies, Palo Alto, CA).
Label biotin
Label protocol biotin, according to manufacturer's recommendations
Hybridization protocol cRNA, according to manufacturer's recommendations
Scan protocol according to manufacturer's recommendations
Data processing RMA; RMA preprocessed log2 expression values
Submission date Apr 02, 2012
Last update date Mar 04, 2013
Contact name Dario Greco
Organization name Tampere University
Department Faculty of Medicine and Health Technology
Lab Finnish Hub for Development and Validation of Integrated Approaches (FHAIVE)
Street address Arvo ylpön Katu 34
City Tampere
ZIP/Postal code 33520
Country Finland
Platform ID GPL15395
Series (1)
GSE36990 Gene expression profile of PBMCs in response to metreleptin treatment at different time points.

Data table header descriptions
VALUE log2 RMA normalized signal

Data table
10000_at 2.809992339
10001_at 4.522588684
10002_at 2.890507118
10003_at 3.517660101
100048912_at 2.839525334
10004_at 3.257163594
10005_at 5.184066279
10006_at 6.855387262
10007_at 4.635849992
10009_at 3.203169465
1000_at 3.194133037
10010_at 5.328968942
100126791_at 3.664155534
100128124_at 2.836525678
100128640_at 3.882399631
100129128_at 2.480965277
100129250_at 6.008778671
100129271_at 4.244849445
100129482_at 4.218000454
100129973_at 2.668627651

Total number of rows: 12139

Table truncated, full table size 242 Kbytes.

Supplementary file Size Download File type/resource
GSM908153_Holly_40.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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